Phenol oxidizing enzyme enzymes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C11D 3/386 (2006.01) C12N 1/14 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 15/80 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2322661

Disclosed herein are phenol oxidizing enzymes obtainable from species of Stachybotrys which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys chartarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts. Disclosed herein are enzyme compositions comprising phenol oxidizing enzymes obtainable from species of Stachybotrys.

L'invention concerne des enzymes oxydant le phénol pouvant être obtenues à partir d'espèces de Stachybotrys, ces enzymes servant à modifier la couleur associée à des colorants et à des composés colorés, et pouvant également être utilisées dans des applications destinées à prévenir tout transfert pigmentaire. L'invention concerne également des cultures biologiquement pures de souches du genre Stachybotrys, ci-après dénommées Stachybotrys parvispora MUSCL 38996 et Stachybotrys chartarum MUCL 38898, lesquelles sont capables de produire naturellement les nouvelles enzymes oxydant le phénol susmentionnées. L'invention concerne en outre la séquence d'acide aminé et la séquence d'acide nucléique de ces nouvelles enzymes oxydant le phénol, ainsi que les vecteurs d'expression et les cellules hôtes contenant cet acide nucléique. L'invention concerne par ailleurs des méthodes destinées à produire ces enzymes oxydant le phénol, ainsi que des méthodes permettant de construire lesdits vecteurs d'expression. L'invention concerne enfin des compositions enzymatiques renfermant lesdites enzymes oxydant le phénol pouvant être obtenues à partir des espèces de Stachybotrys.

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