Phenol oxidizing enzymes and their use

Details Phenol oxidizing enzymes and their use Phenol oxidizing enzymes and their use

C - Chemistry, Metallurgy

11

D

C11D 3/386 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01)

Patent

CA 2323092

Disclosed herein are phenol oxidizing enzymes obtainable from species of Stachybotrys which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys chartarum MUCL 38898, which are capable of naturally producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts. Disclosed herein are detergent compositions comprising phenol oxidizing enzymes obtainable from species of Stachybotrys.

L'invention concerne des enzymes d'oxydation de phénol pouvant être obtenues à partir d'espèces de Stachybotrys et s'utilisant pour modifier la couleur associée aux colorants et composés colorés, ainsi que dans des applications anti-transfert de colorant. L'invention concerne également des cultures biologiquement pures de souches du genre Stachybotrys, désignées ci-après sous le nom de Stachybotrys parvispora MUCL 38996 et Stachybotrys chartarum MUCL 38898, capables de produire naturellement des enzymes d'oxydation de phénol. L'invention concerne aussi la séquence d'acides aminés et la séquence nucléotidique d'enzymes d'oxydation de phénol à partir de Stachybotrys, ainsi que des vecteurs d'expression et des cellules hôtes comprenant l'acide nucléique. L'invention concerne en outre des méthodes de production de l'enzyme d'oxydation de phénol ainsi que des méthodes d'élaboration d'hôtes d'expression. L'invention concerne enfin des compositions détergentes comprenant des enzymes d'oxydation de phénol pouvant être obtenues à partir d'espèces de Stachybotrys.

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