Phenolic acid esterases, coding sequences and methods

Details Phenolic acid esterases, coding sequences and methods Phenolic acid esterases, coding sequences and methods

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 1/21 (2006.01) C12N 9/18 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2340581

Described herein are four phenolic acid esterases, three of which correspond to domains of previously unknown function within bacterial xylanases, from XynY and XynZ of Clostridium thermocellum and from a xylanase of Ruminococcus. The fourth specifically exemplified xylanase is a protein encoded within the genome of Orpinomyces PC-2. The amino acids of these polypeptides and nucleotide sequences encoding them are provided. Recombinant host cells, expression vectors and methods for the recombinant production of phenolic acid esterases are also provided.

L'invention concerne quatre estérases d'acide phénolique dont trois correspondent à des domaines à fonction inconnue auparavant dans des xylanases bactériennes, de XynY et XynZ de Clostridium thermocellum et d'une xylanase de Ruminococcus. La quatrième xylanase citée spécifiquement à titre d'exemple est une protéine codée dans le génome d'Orpinomyces PC-2. Les acides aminés de ces polypeptides et les séquences nucléotidiques les codants sont également décrits ainsi que des cellules hôtes de recombinaison, des vecteurs d'expression et des procédé de production par recombinaison d'estérases d'acide phénolique.

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