Phosphate biosensors and methods of using the same

Details Phosphate biosensors and methods of using the same Phosphate biosensors and methods of using the same

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A01K 67/027 (2006.01) C07K 14/195 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 15/12 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12N 15/65 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2625327

Phosphate biosensors are disclosed, which comprise a phosphate binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon phosphate binding. Such biosensors are useful for real time monitoring of phosphate metabolism in living cells.

La présente invention concerne des bio-capteurs de phosphate qui comprennent un domaine de liaison au phosphate conjugué à des fragments donneurs et fluorescents permettant la détection et le mesurage du transfert d'énergie par résonance en fluorescence après liaison au phosphate. De tels bio-capteurs sont utiles pour la surveillance en temps réel du métabolisme du phosphate dans des cellules vivantes.

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