Phosphate determination

Details Phosphate determination Phosphate determination

G - Physics

01

N

150/3, 150/9

G01N 33/52 (2006.01) G01N 33/84 (2006.01) G01N 33/96 (2006.01)

Patent

CA 1197762

ABSTRACT An aqueous reagent for determining inorganic phosphate in a protein-containing liquid biological sample comprises a molybdate salt, an acid capable of reacting with the molybdate salt to form molybdic acid for complexation with phosphate to form phosphomolybdate complexes, a ferric salt in an amount sufficient to inhibit turbidity in the sample, and a nonionic surfactant reagent in an amount sufficient to further inhibit turbidity in the sample, the molybdate salt and the acid being present in amounts sufficient to form an amount of molybdic acid sufficient to produce a detectable color change when complexed with phosphate in the sample.

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