Phosphatonin-related gene and methods of use thereof

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) C07K 14/575 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01) G01N 33/74 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2416693

This invention provides methods for modulating phosphate homeostasis and renal phosphate transport ba delivering agents that alter the expression of the FRP- 4 gene of alter the activity of the FRP-4 protein. The methods of the invention are useful for modulating bone mineralization, renal phosphate transport, alleviating oncogenic osteomalacia-associated symptoms and treating phosphate homeostasis-related disease. The invention further provides methods for reducing phosphate re-absorption by delivering to a subject FRP-4 protein or polynucleotides that encode this protein. In addition, the invention provides methods for detecting and monitoring expression of the FRP-4 gene and modulating the phenotype of a neoplastic cell associated with oncogenic osteomalacia. Finally, the invention provides methods for screening candidate agents to identify compositions that modify the activity of the FRP-4 gene and protein.

La présente invention concerne des méthodes de modulation de l'homéostasie du phosphate et du transport du phosphate rénal, reposant sur l'administration d'agents qui modifient l'expression du gène FRP-4 ou l'activité de la protéine FRP-4. Les méthodes de l'invention sont utilisées pour moduler la minéralisation osseuse et le transport du phosphate rénal, pour atténuer les symptômes oncogènes associés à l'ostéomalacie, et pour traiter les maladies liées à l'homéostasie du phosphate. Par ailleurs, cette invention concerne des méthodes de réduction de la réabsorption du phosphate consistant à administrer à un sujet de la protéine FRP-4 ou des polynucléotides qui codent cette protéine. De plus, l'invention concerne des méthodes de détection et de surveillance de l'expression du gène FRP-4 et de modulation du phénotype d'une cellule cancéreuse associée à l'ostéomalacie oncogène. Enfin, l'invention concerne des méthodes de criblage d'agents candidats permettant d'identifier des compositions qui modifient l'activité du gène et de la protéine FRP-4.

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