Phosphinotricin-resistance gene, and its use

Details Phosphinotricin-resistance gene, and its use Phosphinotricin-resistance gene, and its use

C - Chemistry, Metallurgy

12

N

195/131, 47/4, 1

C12N 15/54 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/64 (2006.01) C12N 15/65 (2006.01) C12N 15/82 (2006.01) C12P 41/00 (2006.01)

Patent

CA 1337597

Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.

545037

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