Phosphopantetheinyl transferases from bacteria

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/12 (2006.01)

Patent

CA 2638806

The invention relates generally to phosphopantetheinyl transferases that are required for activation of a polyketide synthase complex to synthesize long chain poly-unsaturated fatty acids (LC- PUF A's) such as docosahexaenoic acid and eicosapentaenoic acid. In particular, the invention relates to bacterial phosphopantetheinyl transferases, DNA constructs for their expression in host cells, and seed, oil and meal when the host cells comprise a plant. Also provided is a method for making a plant oil containing docosahexaenoic acid and/or eicosapentaenoic acid.

L'invention concerne généralement des phosphopantéthéinyl transférases qui sont nécessaires pour l'activation d'un complexe de polycétide synthase pour synthétiser des acides gras poly-insaturés à longue chaîne (LC-PUF A) tels que l'acide docosahexaénoïque et l'acide écicosapentanéoïque. En particulier, l'invention concerne des phosphopantéthéinyl transférases, des ADN chimères pour leur expression dans des cellules hôtes, et des graines, de l'huile et de la farine quand les cellules hôtes comprennent une plante. L'invention concerne aussi un procédé pour produire une huile végétale contenant de l'acide docosahexaénoïque et/ou de l'acide éicosapentaénoïque.

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