C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/14 (2006.01) C07K 14/195 (2006.01) C12N 9/16 (2006.01)
Patent
CA 2447033
The present invention provides enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention provides a phosphotriesterase enzyme identified from an Agrobacterium radiobacter strain isolated from soil that hydrolyses OP pesticides, and the gene encoding that enzyme. The invention also provides mutants of the identified phosphotriesterase enzyme which have altered substrate specificity. The use of these enzymes in bioremediation strategies is also provided.
L'invention concerne des enzymes capables d'hydrolyser des molécules d'organophosphate (OP). En particulier, l'invention concerne une phosphotriestérase identifiée dans une souche d'Agrobacterium radiobacter isolée dans le sol et qui hydrolyse les pesticides organophosphorés OP, ainsi que le gène codant cette enzyme. Par ailleurs, l'invention concerne des mutants de l'enzyme phosphotriestérase identifiée qui présentent une spécificité de substrat modifiée. L'invention concerne également l'utilisation de ces enzymes dans des procédés de biorestauration.
Harcourt Rebecca
Horne Irene
Oakeshott John
Russell Robyn
Sutherland Tara
Commonwealth Scientific And Industrial Research Organisation
Sim & Mcburney
LandOfFree
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