Photoimmune detection of dna and rna

Details Photoimmune detection of dna and rna Photoimmune detection of dna and rna

C - Chemistry, Metallurgy

12

Q

167/37

C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 1258624

PHOTOIMMUNE DETECTION OF DNA AND RNA Abstract of the Invention A photoimmune method for detecting a particular polynucleotide sequence in a nucleic acid-containing sample is provided. A single-stranded polynucleotide probe substan- tially complementary to the polynucleotide sequence to be detected is irradiated with ultraviolet light to induce formation of UV-photoproducts, principally pyrimidine dimers. The UV-labelled probe is contacted with denatured nucleic acid from the test sample under hybridizing conditions. Duplex formation is detected with anti-UV-nucleic acid antibodies which bind the irradiated probe. The antibodies are labelled with a signalling means. A method for detect- ing/quantifying total DNA or RNA in a sample is also provided, as are kits for detecting particular nucleotide sequences, or for detecting total nucleic acid.

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