Photoinhibitory promoter

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/11 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 5/14 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2328139

A DNA fragment or a promoter for expressing a target gene specifically under photoinhibitory or dark conditions. A photoinhibitory promoter is obtained from the 5'- upstream region of a plant gene expressed specifically under photoinhibitory or dark conditions and the function of this promoter is analyzed in detail to thereby clarify the cis element sequence and the core sequence participating in the photoinhibitory expression. An expression cassette having a DNA fragment containing these sequences in the upstream of a target gene is constructed and this expression cassette is transferred into a plant cell or a plant. Thus, a plant cell or a plant wherein the target gene is expressed specifically under photoinhibitory or dark conditions can be obtained.

L'invention porte sur un fragment d'ADN d'un promoteur permettant d'exprimer un gène cible spécifiquement dans des conditions photoinhibitrices ou d'obscurité totale. Selon cette invention, on obtient un promoteur photoinhibiteur à partir de la région amont 5' d'un gène de plante exprimé spécifiquement dans des conditions photoinhibitrices ou d'obscurité totale, et on analyse la fonction de ce promoteur en détail de façon à clarifier la séquence de l'élément cis et la séquence centrale participant à l'expression photoinhibitrice. On construit une cassette d'expression dont le fragment d'ADN contient ces séquences en amont d'un gène cible et on la transfère dans une cellule de plante ou dans une plante. On peut ainsi obtenir une cellule de plante ou une plante où le gène cible est exprimé spécifiquement dans des conditions photoinhibitrices ou sombres.

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