G - Physics – 01 – N
Patent
G - Physics
01
N
73/53, 73/51.5
G01N 21/64 (2006.01) G01N 21/77 (2006.01) G01N 27/07 (2006.01) G01N 27/28 (2006.01) G01N 33/543 (2006.01)
Patent
CA 1246891
ABSTRACT A method of optical analysis of a test sample which comprises a sample material with light-absorbing, scattering, fluorescent, phosphorescent or luminescent properties, which sample is partly in a liquid phase and partly bound to an adjacent solid surface, to discriminate the respective parts of said sample material which are located in the liquid and bound to said solid surface: comprising the steps of providing as said solid surface a surface of a transparent solid optical waveguide, and measuring light from the sample material bound to said solid surface that has passed into and through said transparent solid optical waveguide with total internal reflections and emerged from said waveguide at an angle that deviates from the optical axis of said waveguide by an angle appreciably less than ? , where ? = arcsin ?(n22-n12) where n2 is the refractive index of the material of the waveguide and n1 is the refractive index of the adjacent liquid, and excluding from said measurement substantially all light that has emerged from said waveguide at an angle that deviates from said optical axis by ? or more.
483753
Shanks Ian A.
Smith Alan M.
Ares-Serono Inc.
Ridout & Maybee Llp
LandOfFree
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