Photon-triggered luminescent assay

Details Photon-triggered luminescent assay Photon-triggered luminescent assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/66 (2006.01) G01N 21/76 (2006.01) G01N 33/53 (2006.01) G01N 33/532 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2328684

There is provided a process of inducing luminescent emission from a luciferase bioluminescent reaction particularly useful in binding assays. A luciferin/luciferase bioluminescent combination, together with an inactive, caged trigger compound such as a cofactor, is subjected to photonic radiation, so as to release the trigger compound in active form, and thereby cause substantially instantaneous reaction of the active trigger compound so released with the luciferin/luciferase combination, to induce photonic emission which can be detected and measured.

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