Phytase polypeptides

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/55 (2006.01) A23K 1/165 (2006.01) A23L 1/015 (2006.01) A23L 1/03 (2006.01) A23L 1/105 (2006.01) A23L 1/211 (2006.01) A23L 1/305 (2006.01) C12N 9/16 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2408254

The present invention relates to isolated polypeptides having phytase activity, recombinant DNA sequences encoding such polypeptides, methods of producing such polypeptides and the use of said polypeptides in transgenic plants. The invention discloses polypeptides having affinity for the substrate phytate (1, 2, 3, 4, 5, 6 myo-inositol-hexakisphosphate, phytic acid), comprising an amino acid sequence as described by the invention. Furthermore, the invention discloses DNA fragments encoding said polypeptides, and cDNA fragments encoding said polypeptides. The polypeptides of the invention may for example be used as an additive in animal feeds, an additive in food for human consumption or to extract proteins from rice bran. The invention also concerns a transgenic plant or part thereof, wherein said plant or part thereof have been genetically modified to comprise a polypeptide as defined in by the invention.

L'invention concerne des polypeptides isolés possédant une activité phytase, des séquences d'ADN recombiné codant de tels polypeptides, des procédés de production de ces polypeptides, ainsi que l'utilisation de ceux-ci dans des plantes transgéniques. L'invention concerne notamment des polypeptides possédant une affinité pour le substrat phytate (1, 2, 3, 4, 5, 6 myo-inositol-hexakisphosphate, acide phytique, et comprenant une séquence d'acides aminés telle que décrite dans l'invention. En outre, l'invention concerne des fragments d'ADN et d'ADNc, codant pour ces polypeptides. Il est possible d'utiliser les polypeptides de l'invention en tant qu'additif dans l'alimentation animale, en tant qu'additif dans l'alimentation de l'homme, ou pour extraire des protéines à partir du son de riz. L'invention concerne enfin une plante transgénique ou une partie de celle-ci, lesquelles ont été génétiquement modifiées de manière à comprendre un polypeptide tel que défini dans l'invention.

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