Phytase variants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/16 (2006.01)

Patent

CA 2647476

The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

La présente invention concerne une phytase identique au moins à 74 % à une phytase dérivée de Citrobacter braakii, et qui comporte au moins une modification par comparaison à ladite phytase. Ces variants de phytase possèdent des propriétés modifiées, de préférence améliorées, telles que la thermostabilité, le profil de température, le profil de pH, l'activité spécifique, la performance dans les aliments pour animaux, la sensibilité réduite vis-à-vis des protéases, et/ou un mode de glycosylation modifié. L'invention concerne également un ADN codant pour ces phytases, des procédés permettant leur production, ainsi que leur utilisation, par exemple, dans les aliments pour animaux et les additifs d'aliments pour animaux.

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