C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C07K 14/47 (2006.01) C12N 9/02 (2006.01)
Patent
CA 2397494
Transcription promoter and terminator sequences from the <i>Pichia methanolica</i> glyceraldehyde-3-phosphate dehydrogenase 1 gene (<i>GAP1 gene</i>) are disclosed. The sequences are useful within DNA constructs for the production of proteins of interest in cultured. <i>P. methanolica</i> cells. Within the expression vectors, a <i>GAP1</i> promoter and/or a <i>GAP1</i> terminator is operably linked to A DNA segment encoding the protein of interest.
L'invention concerne des séquences de promoteur et de terminateur de transcription du gène de la glycéraldéhyde-3-phosphate-déhydrogénase 1 de <i>Pichia methanolica</i> gène (<i>GAP1</i>). Ces séquences sont utiles dans des constructions d'ADN pour la production de protéines d'intérêt dans des cellules cultivées de <i>P. methanolica</i>. Dans les vecteurs d'expression, un promoteur <i>GAP1</i> et/ou un terminateur <i>GAP1</i> sont reliés de manière fonctionnelle à un segment d'ADN codant pour la protéine d'intérêt.
Miller Brady G.
Raymond Christopher K.
Sloan James S.
Vanaja Erica
Miller Brady G.
Sloan James S.
Smart & Biggar
University Of Washington Board Of Regents
Zymogenetics Inc.
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