Pichia pastoris linear plasmids and dna fragments thereof

Details Pichia pastoris linear plasmids and dna fragments thereof Pichia pastoris linear plasmids and dna fragments thereof

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C12N 15/11 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2078365

Two novel linear DNA plasmids, pPP1 and pPP2, are described. Also, novel fragments of said plasmids containing the autonomous replica- tion sequence (ARS), and thus capable of self-maintenance as extra chrom- osomal elements are provided. These novel DNA sequences of the present invention are useful in a vector for self-maintenance in Pichia pastoris and are also useful as plasmid vectors which can be shuttled between yeast and bacteria.

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