Pig model for psoriasis

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/00 (2006.01) C12N 15/873 (2010.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2715856

The present invention relates to a genetically modified pig as a model for studying psoriasis. The modified pig model displays one or more phenotypes associated with psoriasis. Disclosed is also a modified pig comprising a mutation in the endogenous ILK-I Ra, JunB/cJun, CD18, IKK2, and/or LIG1 gene, and/or a human, porcine and/or murine PPARs, PPAR-.delta., hcB-.alpha., STAT3c, 1ntegrin beta 1, 1ntegrin alpha 2, MEK1, Amphiregulin, BMP-6, VEGF, JunB.DELTA.ec-Jun.DELTA.ep, IL-I a, TGF.beta 1, CD18 hypo, Cre- IIKK2 fl7fl, Dsgl, SCCE, TGF-a, TNF-a, IL-20, IFN-g, LIG1 KO, KGF, IL-6, PAFR1 Cre/1kk2FL/FL, IL1 R, Dsg3, IFN-gamma, p40, ILI Ra, IKK2, JunB/c-Jun, and/or LIG1 gene, transcriptional and/or translational product or part thereof. The invention further relates to methods for producing the modified pig; and methods for evaluating the effect of a therapeutical treatment of psoriasis, for screening the efficacy of a pharmaceutical composition, and a method for treatment of human being suffering from psoriasis are disclosed.

La présente invention porte sur un porc génétiquement modifié servant de modèle pour l'étude du psoriasis. Le modèle porcin modifié présente un ou plusieurs phénotypes associés au psoriasis. L'invention concerne également un porc modifié comprenant une mutation dans le gène ILK-I Ra, JunB/Cjun, CD18, IKK2 et/ou LIG1 endogène, et dans un gène humain, porcin et/ou murin PPAR, PPAR-d, l?B-a, STAT3c, Intégrine bêta 1, Intégrine alpha 2, MEK1, Amphiréguline, BMP-6, VEGF, JunB?ec-Jun?ep, IL-I a, TGF.bêta 1, CD18 hypo, Cre-IIKK2, fl7fl, Dsg1, SCCE, TGF-a, TNF-a, IL-20, IFN-g, LIG1 KO, KGF, IL-6, PAFR1 Cre/lkk2FL/FL, IL1 R, Dsg3, IFN-gamma, p40, ILI Ra, IKK2, JunB/c-Jun et/ou LIG1, un produit transcriptionnel et/ou traductionnel ou une partie de ceux-ci. L'invention porte en outre sur des procédés de production du porc modifié; et sur des procédés d'évaluation de l'effet d'un traitement thérapeutique du psoriasis, de criblage de l'efficacité d'une composition pharmaceutique, et sur un procédé de traitement d'un être humain qui est atteint de psoriasis.

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