Pigment epithelium-derived factor: characterization, genomic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/18 (2006.01) C12N 5/079 (2010.01) A61K 38/18 (2006.01) A61K 48/00 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2190365

Nucleic acids encoding the neuwtrophic protein known as pigment epithelium-derived factor (PEDF), a truncated vension of PEDF referred to as rPEDF, and equivalent proteins, vectors comprising such nucleic acids, host cells into which such vectors have been introduced, recombinant methods for producing PEDF, rPEDF, and equivalent proteins, the rPEDF protein and equivalent proteins of rPEDF and PEDF -BP, -BX and BA, and the PEDF protein produced by recombinant methods. Effects and use of these variants on: 1) neuronal differentiation (neurotrophic effect), 2) neuron survival (neuronotrophic effect), and 3) glial inhibition (gliastatic effect) are described.

L'invention concerne des acides nucléiques codant pour la protéine neurotrophique connue sous le nom de facteur dérivé de l'épithélium pigmentaire (PEDF), une version tronquée du PEDF appelée RPEDF, et des protéines équivalentes, des vecteurs comprenant ces acides nucléiques, des cellules hôtes dans lesquelles ces vecteurs ont été introduits, des procédés de recombinaison pour produire PEDF, rPEDF, et des protéines équivalentes, la protéine de rPEDF et les protéines équivalents de rPEDF et PEDF -BP, -BX et BA, et la protéine de PEDF produite par des procédés de recombinaison. Sont également décrits les effets et les utilisations de ces variantes sur: 1) la différenciation neuronale (effet neurotrophique), 2) la longévité des neurones (effet neuronotrophique) et 3) l'inhibition gliale (effet gliastatique).

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