Pin m iii gene in white pine

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) C07K 14/415 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) C07K 16/16 (2006.01)

Patent

CA 2244195

A gene coding for Pin m III, which is associated with overwintering and frost hardiness in western white pine, has been cloned and characterized. The gene contains an open reading frame of 486 base pairs, encoding a protein of 161. amino acid residues, with a calculated molecular weight of 18002.30 daltons. The predicted isoelectricl point is 5.534. The gene is transcriptionally regulated with the highest expression in fall and winter months. The protein Pin m III is high in infected tissue such as canker. Accordingly, the gene can be used to determine frost hardiness and blister resist fungus susceptibility in conifers.

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