Plant adenylosuccinate lyase and dna coding therefor

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/60 (2006.01) C07H 21/00 (2006.01) C12N 9/88 (2006.01) C12N 15/82 (2006.01) C12Q 1/527 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2205418

The present invention provides novel plant DNA sequences coding for native adenylosuccinate lyase (ADSL). Methods for using the complete or partial ADSL coding sequence as a probe for diagnostic, mapping and other purposes are taught. Generation of transformed host cells capable of expressing ADSL is also taught. Methods of using the transformed host cells are taught, including methods for recombinant production of ADSL enzymes. A method for using the plant ADSL enzyme to screen for inhibitors of ADSL activity is also provided.

La présente invention se rapporte à de nouvelles séquences d'ADN végétal codant pour l'adénylosuccinate lyase native (ADSL), à des procédés d'utilisation de la séquence codant l'ADSL complète ou partielle comme sonde de diagnostic, à des procédés de cartographie et autres. L'invention se rapporte également à la production de cellules hôtes transformées capables d'exprimer l'ADSL, à des procédés d'utilisation des cellules hôtes transformées, y compris des procédés s'appliquant à la production par recombinaison des enzymes ADSL, et à des procédés d'utilisation de l'enzyme ADSL végétale pour cribler des inhibiteurs de l'activité de l'ADSL.

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