Plant adenylosuccinate synthetase and dna coding therefor

Details Plant adenylosuccinate synthetase and dna coding therefor Plant adenylosuccinate synthetase and dna coding therefor

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C07H 21/04 (2006.01) C12N 9/00 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2207024

The present invention provides novel plant DNA sequences coding for native adenylosuccinate synthetase (ADSS). Methods for using the complete or partial ADSS coding sequence as a probe for diagnostic, mapping and other purposes are taught. Generation of transformed host cells capable of expressing ADSS is also taught. Methods of using the transformed host cells are taught, including methods for recombinant production of ADSS enzymes. A method for using the plant ADSS enzyme to screen for inhibitors of ADSS activity is also provided.

La présente invention concerne le codage de séquences d'ADN d'origine végétale pour la synthétase d'adénylosuccinate native ou "ADSS" (ADenylosuccinate SynthetaSe). L'invention concerne également des procédés d'utilisation de tout ou partie de la séquence de codage ADSS comme sonde destinée notamment au diagnostic et à la cartographie. L'invention concerne en outre des générations de cellules hôtes transformées capable d'exprimer l'ADSS. L'invention concerne enfin non seulement des procédés de transformation des cellules hôtes, y compris des procédés pour la production par recombinaison d'enzymes d'ADSS, mais également des procédés d'utilisation d'enzymes issues de l'ADSS d'origine végétale pour le dépistage d'inhibiteurs de l'activité ADSS.

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