C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12N 9/90 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01) C12P 19/34 (2006.01) C12Q 1/26 (2006.01) C40B 30/04 (2006.01)
Patent
CA 2292778
This invention relates to an isolated nucleic acid fragment encoding a plant enzyme that catalyzes steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate epimerase, threonine synthase, threonine deaminase and S- adenosylmethionine synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.
L'invention a trait à un fragment d'acide nucléique isolé qui code pour une enzyme végétale catalysant des étapes de la biosynthèse de lysine, thréonine, méthionine, cystéine et isoleucine provenant d'aspartate, l'enzyme comportant un élément sélectionné dans le groupe constitué par: la dihydrodipicolinate réductase, la diaminopimélate épimérase, la thréonine synthase, la thréonine désaminase et la S-adénosylméthionine synthétase. L'invention a également trait à la constitution d'un gène chimère codant pour tout ou partie de l'enzyme, dans une orientation sens ou antisens, l'expression du gène chimère ayant pour résultat une production de taux modifiés de l'enzyme dans une cellule hôte transformée.
Abell Lynn Marie
Allen Stephen M.
Falco Saverio Carl
Hitz William D.
Kinney Anthony John
E.i. Du Pont de Nemours And Company
Torys Llp
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