Plant and viral promoters

Details Plant and viral promoters Plant and viral promoters

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01H 5/00 (2006.01) C07K 14/01 (2006.01) C12N 5/14 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2295301

The invention provides plant and viral promoters that can be used to confer high-level gene expression on transgenic plants. Representative promoters can be isolated from the genome of Australian banana-infecting badnaviruses from cultivars Mysore, Williams and Goldfinger. The invention further provides constructs which include the disclosed promoters operatively linked to a coding sequence. Still further provided are a method of expressing the product of a gene in a plant cell, plant cells having DNA constructs within their genomes, and plants comprising the transgenic cells.

On peut faire appel à ces promoteurs végétaux et viraux pour conférer une expression génique supérieure à des plantes transgéniques. Il est possible d'isoler, à partir du génome de virus bacilliformes à ADN infectant la banane d'Australie et issus de cultivars du type Mysore, Williams et Goldfinger, des promoteurs exemplaires. L'invention, qui concerne, de surcroît, des produits de recombinaison renfermant les promoteurs de l'invention liés fonctionnellement à une séquence codante, porte également sur des méthodes visant à l'expression du produit d'un gène dans une cellule végétale, sur des cellules dont les génomes renferment des produits de recombinaison d'ADN ainsi que sur des plantes possédant les cellules transgéniques.

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