Plant artificial chromosomes, uses thereof and methods of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 1/04 (2006.01) C07H 21/00 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/29 (2006.01) C12N 15/63 (2006.01) C12N 15/65 (2006.01) C12N 15/90 (2006.01) C12P 19/34 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2448096

Methods for preparing cell lines that contain plant artificial chromosomes, methods for preparation of plant artificial chromosomes, methods for targeted insertion of heterologous DNA into plant artificial chromosomes, and methods for delivery of plant chromosomes to selected cells and tissues are provided. In particular, plant artificial chromosomes that are substantially composed of repeated nucleic acid units of varying amounts of heterochromatin and euchromatin are provided. Also provided are methods of using plant and animal artificial chromosomes in the production of valuable transgenic plants. Methods for identifying plant genes encoding particular traits using artificial chromosomes and for producing an acrocentric plant chromosome are also provided.

L'invention concerne des lignées cellulaires qui contiennent des chromosomes artificiels de plantes. L'invention a aussi pour objet des procédés de préparation de chromosomes artificiels de plantes, des procédés pour l'insertion ciblée d'ADN hétérologue dans des chromosomes artificiels de plantes, et des procédés pour administrer des chromosomes de plantes à des cellules et des tissus sélectionnés. En particulier, l'invention traite de chromosomes artificiels de plantes qui sont sensiblement composés d'unités d'acide nucléique répétées de quantités diverses d'hétérochromatine et d'euchromatine. Enfin, l'invention a aussi pour objet des procédés d'utilisation de chromosomes artificiels de plantes et d'animaux dans la production de plantes transgéniques intéressantes. L'invention concerne enfin des procédés permettant d'identifier des gènes de plantes codant des caractéristiques particulières à l'aide de chromosomes artificiels et de produire un chromosome de plante acrocentrique.

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