C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) A01H 1/00 (2006.01) A01H 3/00 (2006.01) A01H 5/00 (2006.01) C07H 21/00 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2408223
The objective of the present invention is to isolate and identify genes responsive to plant hormones, such as, brassinolide, and to provide these genes and their use. The present inventors found two novel genes (referred to as OsBLE1 gene and OsBLE2 gene) whose expressions are markedly increased by brassinolide and auxin, using DNA microarray techniques and Northern blotting. Transformed rice plants were produced using Agrobacterium EHA101 which comprise antisense polynucleotide against OsBLE1 and OsBLE2 under the control of the CaMV35S promoter in a binary vector pIG121-Hm. As a result, the transformed rice plants exhibited inhibition in stem and leaf growth as compared with controls which carried the vector alone.
Komatsu Setsuko
Yang Guangxiao
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Bio-Oriented Technology Research Advancement Institution
National Agriculture And Bio-Oriented Research Organization
National Institute Of Agrobiological Sciences
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