Plant cell death system

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01)

Patent

CA 2425321

This invention seeks to provide means by which transgenic plants harbour within their genome a chimaeric gene which when expressed produces a protein having a cytotoxic effect. Cytoxicity is achieved by way of a protein or part thereof capable of blocking cellular protein synthesis, such as a maize ribosome inactivating protein or part thereof. The chimaeric gene may comprise a promoter operably linked to a sequence encoding a protein capable of blocking cellular protein synthesis such that the promoter is inducible at and/or adjacent to a target site.

Cette invention vise à permettre à des plantes transgéniques de loger au sein de leur génome un gène chimérique qui, lorsqu'il s'exprime, produit une protéine présentant un effet cytotoxique. On obtient une cytotoxicité au moyen d'une protéine ou d'une partie de proteine pouvant neutraliser une synthèse de protéine cellulaire, telle qu'une protéine ou partie de protéine neutralisante de ribosome de maïs. Le gène chimérique peut comporter un promoteur lié de manière fonctionnelle à une séquence codant une protéine pouvant neutraliser une synthèse de protéine cellulaire de sorte que le promoteur est inductible dans une zone cible et/ou à proximité adjacente de ladite zone.

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