Plant cell microinjection technique

Details Plant cell microinjection technique Plant cell microinjection technique

C - Chemistry, Metallurgy

12

N

195/1.36

C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12N 15/89 (2006.01)

Patent

CA 1280081

24 PLANT CELL MICROINJECTION TECHNIQUE Abstract of the Invention Microinjection techniques for plant proto- plasts utilize a holding pipette for immobilizing the protoplast while an injection pipette is utilized to inject the macromolecule. In order to manipulate the protoplasts without damage, the protoplasts are cultured for from about 1 to 5 days before the injection is per- formed to allow for partial regeneration of the cell wall. It was found that injection through the partially regenerated cell wall could still be accomplished and particular compartments of the cell could be targeted. The methods are particularly useful for transformation of plant protoplasts with exogenous genes. CCCCC7/8679-9Rev

489269

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