Plant chitinase gene and use thereof

Details Plant chitinase gene and use thereof Plant chitinase gene and use thereof

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) A01N 63/00 (2006.01) A01N 63/02 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2048696

ABSTRACT A DNA sequence comprising the sugar beet chitinase 4 DNA sequence shown in Sequence 1 or an analogue or subsequence thereof is disclosed The polypeptide encoded by the DNA sequence, also termed the sugar beet chitinase 4 enzyme, has a high antifungal activity due to a hitherto unreported bifunctional catalytic activity (i.e. a chitinase and a lysozyme activity) which makes the enzyme highly effective in inhibiting the growth of chitin-containing fungi. An even improved antifungal effect is obtained when the sugar beet sugar beet chitinase 4 enzyme is used in combination with other pathogenesis related proteins, especially in combination with a second different chitinase and a .beta.-1,3-glucanase. A preferred use of the DNA sequence disclosed herein, optionally in combination with DNA sequences encoding other pathogenesis related proteins, is in the construction of genetically transformed plants, especially genetically transformed sugar beet plants, having an increased resistance to chitin-containing fungi as compared to untransformed plants.

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