Plant derived hydroxy phenyl pyruvate dioxygenases (hppd)...

Details Plant derived hydroxy phenyl pyruvate dioxygenases (hppd)... Plant derived hydroxy phenyl pyruvate dioxygenases (hppd)...

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01N 61/00 (2006.01) C07K 14/415 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 15/82 (2006.01) C12Q 1/26 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2427787

An HPPD-inhibitor resistant HPPD enzyme comprising an amino acid sequence GIKECQ and a sequence F, (D/E), F, (M/L), W1, (P/A), P, W2, X, X, Y, Y wherein W1 is either A or P and where (i) if W1 is A then W2 is P, A, Q or L or, (ii) if W1 is P then W2 is P, A, Q or T, and wherein X is any amino acid. The invention also include methods of identifying HPPD inhibitor resistant HPPD enzymes and also of the enzymes thus identified.

L'invention concerne une enzyme HPPD résistante aux inhibiteurs de HPPD, qui comporte une séquence d'acides aminés GIKECQ et une séquence F, (D/E), F, (M/L), W1, (P/A), P, W2, X, X, Y, Y, dans laquelle W1 représente soit A soit P, et (1) si W1 représente A alors W2 représente P, A, Q ou L, ou (2) si W1 représente P alors W2 représente P, A, Q ou T, et X représente n'importe quel acide aminé. L'invention concerne aussi des procédés d'identification d'enzymes HPPD résistantes aux inhibiteurs de HPPD, ainsi que les enzymes ainsi identifiées.

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