Plant-derived resistance gene

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) A01H 1/00 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) C07H 21/04 (2006.01) C07K 14/415 (2006.01) C07K 16/16 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2459079

Disclosed are nucleic acid molecules encoding polypeptides which are capable of conferring resistance against, and being triggered by, plant pathogens such as fungi (e.g. Phytophtora infestans and related isolates). Preferred nucleic acid molecules encode R1 from Solanum tuberosum, or a variety of homologs, naturally occurring or derivatives) thereof. Particular methods of activating resistance by using the R1 resistance gene are disclosed, which in certain cases lead to a hypersensitive response. Further aspects of the invention include specific primers, vectors, host cells, polypeptides, antibodies, aptamers, transgenic plants, methods of producing and employing these, and methods of influencing the resistance trait in a plant. Furthermore, screening methods for identifying and obtaining plant protective compounds are provided.

L'invention concerne des molécules d'acide nucléique codant pour des polypeptides capables de conférer une résistance contre des pathogènes de plantes tels que les mycoses (p. ex. Phytophtora infestans et isolats apparentés) et d'être activés par ces derniers. Les molécules d'acide nucléique préférées codent pour R1 de Solanum tuberosum, ou divers homologues naturels ou dérivés de ceux-ci. L'invention concerne également des procédés particuliers d'activation de la résistance comprenant l'utilisation du gène de résistance R1, et qui dans certains cas conduisent à une réaction hypersensible. L'invention concerne en outre des amorces, des vecteurs, des cellules hôtes, des polypeptides, des anticorps, des aptamères, des plantes transgéniques, des procédé de production et d'utilisation de ceux-ci et des procédés permettant d'influencer la caractéristique de résistance d'une plante. L'invention concerne de plus des procédés de criblage permettant d'identifier et d'obtenir des composés phytosanitaires.

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