Plant fatty acid desaturases and alleles therefor

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 9/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2386111

In one aspect, the invention provides new variants of the Fad3 enzyme, including amino acid substitutions, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention include transgenic plants and plant parts. Vectors capable of transforming plant cells are provided, including the nucleic acids of the invention, including Fad3 coding sequences. Corresponding methods are provided for obtaining the transgenic plants of the invention. Methods are provided for using the plants of the invention, including selected plants and transgenic plants, to obtain plant products. Amplification primers for identifying the Fad3 alleles of the invention are provided, together with methods of obtaining plants using the Fad3 alleles of the invention as markers.

Selon une de ses réalisations, l'invention se rapporte à de nouveaux allèles de l'enzyme Fad3 comportant des substitutions d'acides aminés, ainsi qu'à des séquences nucléotidiques codant pour ces peptides. D'autres aspects de l'invention concernent des plantes transgéniques et des fragments végétaux. L'invention concerne également des vecteurs capables de transformer des cellules végétales et incluant les acides nucléiques de l'invention, et notamment les séquences codant pour Fad3. L'invention se rapporte à des méthodes correspondantes d'obtention des plantes transgéniques décrites ci-dessus. Elle se rapporte également à des procédés d'utilisation desdites plantes, et notamment de plantes sélectionnées et de plantes transgéniques, pour l'obtention de produits végétaux. L'invention se rapporte également à des amorces d'amplification permettant d'identifier les allèles de Fad3, ainsi qu'à des méthodes d'obtention de plantes utilisant les allèles de Fad3 en tant que marqueurs.

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