Plant fatty acid hydroxylase genes

Details Plant fatty acid hydroxylase genes Plant fatty acid hydroxylase genes

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2309791

Several cytochrome P450-dependent fatty acid hydryoxylases from different plant sources have been identified by recombinant cloning technology and characterized structurally and functionally. These clones represent novel plant hydroxylases which are active when expressed in a heterologous yeast system. These hydroxylase enzymes hydroxylate fatty acid substrates at different, well-defined postions in acid substrates of various chain lengths. The hydroxylases catalyze epoxidation of fatty acids, natural and synthetic, bearing a double bond at the site of attack.

Il a été identifié plusieurs hydroxylases d'acide gras dépendantes du cytochrome P450 provenant de différentes sources végétales grâce à des techniques de clonage de recombinaison, ces hydroxylases ayant été ensuite caractérisées structurellement et fonctionnellement. Ces clones représentent de nouvelles hydroxylases végétales qui sont actives une fois exprimées dans un système hétérologue de levure. Ces enzymes hydroxylases, qui hydroxylent des substrats d'acide gras à des positions différentes et bien définies dans des substrats d'acide de longueurs de chaîne variées, catalysent l'époxydation d'acides gras naturels et synthétiques, portant une double liaison sur le site d'attaque.

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