C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01) C12Q 1/26 (2006.01)
Patent
CA 2256501
The invention relates to the isolation and modification of nucleic acid sequences encoding p-hydroxyphenylpyruvate dioxygenase enzyme from plants. These nucleic acid sequences were used to establish methods of identification of new herbicidal compounds that inhibit the activity of this enzyme, and to prepare new crop plants that are tolerant to the herbicidal action of inhibitors of this enzyme. Chimeric genes comprising nucleic acid fragments containing all or part of the nucleic acid sequences encoding p- hydroxyphenylpyruvate dioxygenase may be used to produce active plant p- hydroxyphenylpyruvate dioxygenase enzyme in microorganisms, and to cause the production of modified forms of the enzyme in plants that may render such plants tolerant to inhibitors of the enzyme.
L'invention concerne l'isolement et la modification des séquences nucléotidiques codant l'enzyme p-hydroxyphénylpyruvate dioxygénase de plantes. Ces séquences nucléotidiques ont été utilisées pour établir des procédés d'identification de nouveaux composés herbicides inhibant l'activité de cette enzyme, et pour produire de nouvelles plantes cultivées tolérant l'action désherbante des inhibiteurs de cette enzyme. Des gènes chimères comportant des fragments d'acide nucléique contenant tout ou partie des séquences nucléotidiques codant pour la p-hydroxyphénylpyruvate dioxygénase peuvent être utilisés pour produire l'enzyme active p-hydroxyphénylpyruvate dioxygénase dans des micro-organismes, et pour provoquer la production de formes modifiées de cette enzyme dans des plantes, ces dernières pouvant rendre lesdites plantes tolérantes aux inhibiteurs de cette enzyme.
Gutteridge Steven
Maxwell Carl Arthur
Scolnik Pablo Ariel
Wittenbach Vernon Arie
Bennett Jones Llp
E.i. Du Pont de Nemours And Company
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