C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2409172
Methods and compositions are presented for the generation of targeted alterations in a plant genome using double-stranded homogeneous oligonucleotides containing a single type of nucleotide. These methods can be used to correct mutations, introduce mutations and/or alter gene activity in a plant cell. A cell-free assay system for monitoring genetic alteration using the oligonucleotides of the invention is also presented.
La présente invention concerne des procédés et des compositions permettant de produire des altérations ciblées dans un génome végétal à l'aide d'oligonucléotides homogènes à double brin contenant un seul type de nucléotide. Les procédés de l'invention peuvent être utilisés pour corriger des mutations, introduire des mutations et/ou altérer l'activité génique dans une cellule végétale.L'invention se rapporte également à un système d'analyse acellulaire permettant de contrôler l'altération génétique à l'aide des nucléotides de l'invention.
Kmiec Eric B.
May Gregory D.
Rice Michael C.
Smart & Biggar
University Of Delaware
LandOfFree
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