Plant genomic dna flanking spt event and methods for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2715564

Compositions and methods related to transgenic plants comprising seed production technology are provided. Specifically, maize plants having a E6611.32.1.38 event which confers seed production technology are provided. The plant harboring the E6611.32.1.38 event at the recited chromosomal location comprises the genomic/transgene junctions described. The plant genomic DNA flanking the integrated E6611.32.1.38 event can be used to design assays that will be specific for the E6611.32.1.38 event. The characterization of the genomic insertion site of the E6611.32.1.38 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the maize E6611.32.1.38 event are provided.

La présente invention concerne des compositions et des procédés relatifs à des plantes transgéniques, dont une technologie de production de semences. Plus précisément, la présente invention concerne des plants de maïs porteurs de l'événement E6611.32.1.38 à l'origine d'une technologie de production de semences. La plante porteuse de l'événement E6611.32.1.38 au niveau de l'emplacement chromosomique précisé comprend les jonctions génomiques/transgéniques décrites. L'ADN génomique végétal flanquant l'événement E6611.32.1.38 intégré peut être utilisé pour la conception d'analyses spécifiques de l'événement E6611.32.1.38. La caractérisation du site d'insertion génomique de l'événement E6611.32.1.38 assure une efficacité reproductive accrue et permet l'utilisation de marqueurs moléculaires permettant de retrouver le transgène inséré dans les populations se reproduisant et dans leur descendance. L'invention concerne, encore, divers procédés et compositions permettant l'identification, la détection et l'utilisation de l'événement E6611.32.1.38 du maïs.

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