Plant lysophosphatidic acid acyltransferases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 5/00 (2006.01) C11B 1/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2186607

This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are described.

L'invention porte sur des acyltransférases végétales de l'acide lysophosphatidique (LPAAT), des moyens d'identification de ces mêmes protéines, des séquences d'acides aminés et nucléotidiques associées, et leurs procédés d'obtention, de production et d'utilisation. Elle porte également sur leur méthode de purification, et en particulier sur l'élimination des membranes végétales et leur séparation d'avec les autres protéines végétales, ainsi que sur l'emploi desdites LPAAT notamment comme outil d'isolement de gènes dans des applications biotechnologiques. Sont également présentées, des séquences nucléotidiques codant pour certaines régions des LPAAT, et leur utilisation en vue de l'isolement de gènes de LPAAT à partir de végétaux et la modification de compositions de triglycérides végétaux.

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