Plant pathogen resistance genes and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2218554

The tomato Cf-4 gene has been isolated by positional cloning and its sequence provided, along with the encoded amino acid sequence. DNA encoding the polypeptide, alleles, mutants and derivatives thereof, and DNA encoding amino acid sequences showing a significant degree of homology thereto may be introduced into plant cells and the encoded polypeptide expressed, conferring pathogen resistance on plants comprising such cells and descendants thereof. The Cf-4 sequence shows a high degree of homology to Cf-9 and comprises leucine rich repeats.

On a pu isoler le gène Cf-4 de la tomate par clonage positionnel et déterminer sa séquence ainsi que la séquence d'acide aminé codée, l'ADN codant pour le polypeptide, les allèles, les mutants et leurs dérivés et l'ADN codant pour les séquences d'acides aminés et présentant un degré significatif d'homologie avec eux peuvent être introduits dans des cellules végétales; le polypeptide codé est alors exprimé, ce qui confère aux plantes contenant lesdites cellules et à leur descendance une résistance aux éléments pathogènes. La séquence Cf-4 qui présente un degré élevé d'homologie avec la séquence Cf-9 comprend des répétitions riches en leucines.

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