Plant pathogen resistance genes and uses thereof

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C07K 14/37 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2216406

The Arabidopsis RPP5 gene has been cloned and its sequence provided, along with the encoded amino acid sequence. DNA encoding the polypeptide, and alleles, mutants and derivatives thereof, may be introduced into plant cells and the encoded polypeptide expressed, conferring pathogen resistance on plants comprising such cells and descendants thereof. The RPP5 sequence comprises leucine rich repeats and the presence of such repeats enables identification of other plant pathogen resistance genes. Homologies between RPP5 and other pathogen resistance genes reveal motifs useful in identification of other pathogen resistance genes.

Le gène Arabidopsis RPP5 a été cloné et sa séquence a été décrite ainsi que la séquence d'aminoacides codée. L'ADN codant le polypeptide, et ses allèles, mutants et dérivés, peuvent être introduits dans les cellules des plantes et le polypeptide codé peut être exprimé, conférant ainsi aux plantes une résistance aux pathogènes comprenant de telles cellules ainsi que leur descendances. La séquence RPP5 comporte des répétitions riches en leucine et la présence de ces répétitions permet l'identification d'autres gènes de résistance aux pathogènes chez les plantes. Les homologies entre le RPP5 et d'autres gènes de résistance aux pathogènes révèlent des motifs utilisés dans l'identification d'autres gènes de résistance aux pathogènes.

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