Plant stearoyl-acp thioesterase sequences and methods to...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) A01H 5/10 (2006.01) C11B 1/00 (2006.01) C12N 9/16 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2205657

This invention relates to plant thioesterases, specifically plant acyl-ACP thioesterases having significant activity on stearoyl-ACP. DNA constructs useful for the expression of a plant stearoyl-ACP thioesterase in a plant seed cell are described. Such constructs will contain a DNA sequence encoding the plant stearoyl-ACP thioesterase of interest under the control of regulatory elements capable of preferentially directing the expression of the plant stearoyl-ACP thioesterase in seed tissue, as compared with other plant tissues, when such a construct is expressed in a transgenic plant. This invention also relates to methods of using a DNA sequence encoding a plant stearoyl-ACP thioesterase for the modification of the stearate produced in a plant seed cell. A plant thioesterase having significant activity on stearoyl- ACP exemplified herein is from mangosteen. Modified stearate concentration may be obtained by expression of mangosteen thioesterase alone or in combination with antisense constructs for reduction of native stearoyl-ACP desaturase in plant seeds.

L'invention concerne les thioestérases végétales, en particulier des acyl-PVA thioestérases ayant une activité significative sur la stéaroyl-PVA. On décrit des constructions d'ADN utiles pour l'expression d'une stéaroyl-PVA thioestérase végétale dans des graines de plante. Ces constructions vont contenir une séquence d'ADN codant pour la stéaroyl-PVA thioestérase végétale choisie sous le contrôle d'éléments régulateurs capables de diriger préférentiellement l'expression de la stéaroyl-PVA thioestérase végétale vers des tissus de graines, plutôt que d'autres tissus de plante, quand une telle construction est exprimée dans une plante transgénique. Cette invention concerne également des procédés d'utilisation d'une séquence d'ADN codant une stéaroyl-PVA thioestérase de plante pour la modification d'un stéarate produit dans une cellule de graine de plante. Une thioestérase de plante ayant une activité significative sur la stéaroyl-PVA donnée ici comme exemple vient de mangoustan. La concentration modifiée de stéarate peut être obtenue par l'expression de la thioestérase de mangoustan seule ou en combinaison avec des constructions antisense diminuant la stéaroyl-PVA désaturase dans les graines de plantes.

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