Plant transformation method using agrobacterium species

C - Chemistry – Metallurgy – 12 – N

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C12N 15/84 (2006.01) A01H 5/00 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2053473

Plant cells are transformed by bombarding them with microparticles in a typical particle gun, and then treating the bombarded cells with bacteria of the genus Agrobacterium which have been transformed to incorporate the DNA sequence of interest into their T-DNA. High frequencies of stable transformation are achieved.

Les cellules sont transformées en les bombardant avec des microparticules dans un canon à particules, puis en traitant les cellules bombardées avec des bactéries du genre Agrobacterium qui ont été transformées pour incorporer, dans leur AND-T, la séquence d'ADN pertinente. On obtient de hautes fréquences de transformation stable.

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