Plant transformation using dna minicircles

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01)

Patent

CA 2749440

The invention provides methods and compositions for producing and using minicircle DNA molecules that are useful for plant transformation. The invention also provides methods for transforming plant cells and plants with such minicircle DNA molecules, plant cells and plants produced by such methods, and plants transformed with minicircle DNA molecules. The methods and compositions of the invention are particularly useful for producing "intragenic plants" which do not contain any non-native DNA.

La présente invention concerne des procédés et des compositions permettant de produire et d'utiliser des molécules d'ADN minicercle permettant des transformations végétales. L'invention concerne également des procédés de transformation de cellules végétales et de plantes au moyen desdites molécules d'ADN minicercle, des cellules végétales et des plantes produites par lesdits procédés et, également, des plantes transformées au moyen de molécules d'ADN minicercle. Les procédés et compositions de la présente invention se révèlent particulièrement utiles pour la production de "plantes intragéniques" ne contenant aucun ADN non natif.

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