Plant transformation vectors

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2353077

Vectors for transforming plants with the use of agrobacteria which have been modified so as to elevate the possibility of the recognition of the border sequences of the vectors by vir proteins of the agrobacteria, thereby lowering the possibility of the transfer of DNAs other than T-DNA into plant chromosomes. More particularly, the above-vectors are those to be used in transforming plants which have right and left border sequences which can be recognized by the vir proteins of the agrobacteria, a T-DNA sequence which is located between these border sequences and into which a gene to be transferred into plants can be inserted, and a replication origin enabling the replication of the vectors in bacteria, characterized by having a plural number of left border sequences.

L'invention concerne des vecteurs de transformation de plantes, au moyen d'agrobactéries que l'on a modifiées de façon à élever la possibilité de reconnaissance, par les protéines vir des agrobactéries, des séquences de bordure des vecteurs, ce qui contribue à diminuer la possibilité de transfert, dans les chromosomes des plantes, d'ADN autres que l'ADN-T. L'invention concerne notamment des vecteurs qui sont utiles pour transformer des plantes, possèdent des séquences gauche et droite de bordure pouvant être reconnues par les protéines vir des agrobactéries, une séquence d'ADN-T située entre ces séquences de bordure et dans laquelle il est possible d'insérer un gène à transférer dans les plantes, ainsi qu'une origine de réplication permettant la réplication des vecteurs dans les bactéries, et dont la caractéristique est de comporter plusieurs séquences de bordure gauche.

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