Plant transformation with in vivo assembly of a trait

Details Plant transformation with in vivo assembly of a trait Plant transformation with in vivo assembly of a trait

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01)

Patent

CA 2513311

A process of endowing a plant or plant cells with a trait of interest by expressing an RNA sequence of interest, said process comprising: providing plant cells or cells of said plant with a first vector and a second vector and selecting cells endowed with said trait of interest, wherein said first vector contains a first nucleotide sequence with a first segment coding, in 5' to 3' direction, for - a 5' part of said RNA sequence of interest and - a 5' part of an intron; and said second vector contains a second nucleotide sequence with a second segment coding, in 5' to 3' direction, for - a 3' part of an intron and - a 3' part of said RNA sequence of interest.

L'invention concerne un procédé consistant à doter une plante ou des cellules de plantes d'un trait d'intérêt par expression d'une séquence d'ARN d'intérêt, ledit procédé consistant: à doter les cellules de plantes ou les cellules de ladite plante d'un premier vecteur et d'un second vecteur et à sélectionner les cellules dudit trait d'intérêt, ledit premier vecteur contenant une première séquence nucléotidique ayant un premier segment codant, dans le sens 5' à 3' pour une partie 5' de ladite séquence d'ARN d'intérêt et une partie 5' d'un intron; et ledit second vecteur contient une seconde séquence nucléotidique ayant un second segment codant, dans le sens 5' à 3', pour une partie 3' d'un intron et une partie 3' de ladite séquence d'ARN d'intérêt.

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