C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) A01H 5/00 (2006.01) A61K 31/728 (2006.01) C08B 37/08 (2006.01) C12N 5/10 (2006.01) C12P 19/04 (2006.01)
Patent
CA 2533925
A method of synthesizing hyaluronic acid which comprises: (1) the step of transforming a plant cell or a plant by (i) a DNA encoding hyaluronate synthase, or (ii) a DNA encoding a polypeptide that has an amino acid sequence derived from the amino acid sequence of hyaluronic acid synthase by deletion, substitution, addition or insertion of one or more amino acids and having an activity of synthesizing hyaluronic acid; (2) the step of growing the transformant obtained by the transformation; and (3) the step of separating the hyaluronic acid thus produced.
L'invention porte sur un procédé de synthèse de l'acide hyaluronique consistant: (1) à transformer des cellules végétales ou des plantes à l'aide: (i) soit d'ADN codant pour l'hyaluronate synthase, (ii) soit d'ADN codant pour un polypeptide présentant une séquence d'acides aminés de la synthase de l'acide hyaluronique par suppression, substitution, addition ou insertion d'un ou plusieurs acides aminés, et présentant une activité de synthèse de l'acide hyaluronique; (2) à faire croître le transformant résultant de cette transformation; et (3) à séparer l'acide hyaluronique ainsi produit.
Ihara Izumi
Kitazawa Hiroaki
Misawa Shuhei
Shibatani Shigeo
Marks & Clerk
Toyo Boseki Kabushiki Kaisha
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