C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2046646
Two new Bacillus thuringiensis strains; which are deposited at the DSM under accession nos. 5131 and 5132, produce crystal proteins during sporulation that are toxic to Coleoptera. The crystal proteins contain 74 kDa and 129 kDa protoxins, respectively, which can yield 68 and 66 kDa toxins, respectively, as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that codes for its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Coleoptera.
De nouvelles souches de Bacillus thuringiensis, qui sont enregistrées auprès du DSM sous les numéros de dépôt 5131 et 5132, produisent pendant la sporulation des protéines cristallines ayant une action toxique sur les coléoptères. Ces protéines cristallines contiennent des protoxines de 74 kDa et 129 kDa, respectivement, qui peuvent produire des toxines de 68 et 66 kDa, respectivement, comme produits de digestion de trypsine. Une plante, dont le génome transformé au moyen d'une séquence d'ADN provenant de l'une des deux souches et codant pour sa toxine respective, résiste aux coléoptères. Chaque souche, ou ses cristaux, ses protéines cristallines, sa protoxine ou sa toxine, peuvent être utilisés comme ingrédient actif dans une composition insecticide contre les coléoptères.
Joos Henk
Lambert Bart
Peferoen Marnix
Aventis Cropscience N.v.
Goudreau Gage Dubuc
LandOfFree
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