Plants with reduced glucosinolate content

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2235748

The present invention relates to a method to reduce glucosinolate production and/or accumulation in plants wherein a reduced glucosinolate content is desired, particularly in the seeds of these plants. In accordance with the present invention, chimeric gene constructs inhibiting an enzyme responsible for glucosinolate production, the enzyme UDP-glucose:thiohydroximate S- glucosyltransferase (further referred to as "S-GT") are provided. Further in accordance with the present invention, plants are provided having substantially lower glucosinolate levels in their tissues, particularly in their seeds, which permit a significant expansion of the germplasm basis for the breeding of new oilseed rape varieties having lower glucosinolate levels, particularly in their seeds.

La présente invention concerne un procédé de réduction de la production et/ou de l'accumulation de glucosinolate dans des plantes dans lesquelles on désire obtenir une teneur réduite en glucosinolate, notamment dans les graines de celles-ci. Selon la présente invention, on décrit des gènes chimériques de recombinaison inhibant une enzyme responsable de la production de glucosinolate, l'enzyme UDP-glucose:thiohydroximate S-glucosyltransférase (ci-après dénommée "S-GT"). On décrit en outre, conformément à la présente invention, des plantes présentant, dans leurs tissus et notamment dans leurs graines, des concentrations sensiblement plus faibles en glucosinolate, ce qui permet une expansion importante du matériel génétique pour la culture de nouvelles variétés de colza oléagineux présentant des concentrations plus faibles en glucosinolate, notamment dans les graines.

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