Plasma binding protein assay method

Details Plasma binding protein assay method Plasma binding protein assay method

G - Physics

01

N

G01N 33/534 (2006.01) C12Q 1/00 (2006.01) G01N 33/60 (2006.01) G01N 33/96 (2006.01)

Patent

CA 2317874

Disclosed is a method for detection and measurement of plasma binding activity of a test compound in a sample. The method comprises forming a mixture comprising a sample of said test compound in the presence of a plasma binding protein and a radiolabelled ligand; incubating the mixture in the presence of a solid phase under conditions to cause at least a portion of the radiolabelled ligand to become associated with the solid phase and wherein said solid phase comprises a scintillant; and detecting the amount of radiolabelled ligand associated with the solid phase as an indication of plasma protein binding activity of the test compound. Also disclosed is a test kit for the determination the plasma protein binding activity of a test compound in a sample.

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