Plasmid and microbial cell for production of an inhibitory...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/47 (2006.01) C12N 1/21 (2006.01) C12N 15/72 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2067237

- 36 - ABSTRACT A plasmid for the production of an inhibitory protein for phospholipase A2 originated from inflammatory sites, a microbial cell transformed with the plasmid, the protein, and a method of producing same by culturing with the cell, and a pharmaceutical composition for a suppression of allergic reactions comprising same as an active ingredient, are provided. The plasmid comprises a DNA sequence encoding an inhibitory protein for phospholipase A2 originated from inflammatory sites of rat and human, and a DNA sequence that acts as a promotor to regulate the expression of the protein. An Eschericia coli cell is mainly used as the host cell.

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