Plasmid for expression of penicillin g amidase

Details Plasmid for expression of penicillin g amidase Plasmid for expression of penicillin g amidase

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.3

C12N 15/00 (2006.01) C12N 1/20 (2006.01) C12N 9/84 (2006.01)

Patent

CA 1283068

ABSTRACT Plasmids for the increased produciton of penicillin G amidase The present invention provides a plasmid suitable for the expression of penicillin G amidase, wherein it carries an incomplete penicillin G amidase gene in which the first 78 bases of the translated region on t he 5' end of t he complete gene are missing. This incomplete penicillin G amidase gene can also be present incorpor- ated in the form of two separate fragments which commence with bases 79 and 868, respectively, counting from the translated region on the 5' end of the gene and displaying a deletion of the coding bases 706 to 867. The present invention also provides process for the production of these plasmids and is also concerned with the use thereof for obtaining penicillin G amidase.

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